We currently have the following equipment:

• Fluorescence microscope with digital image acquisition system. It has dry and oil immersion objectives (63x and 100x) and filters for DAPI/CMAC, FITC/GFP, CFP, YFP, DsRed and Rodamina visualisation.
• Multidimensional microscopy station. Allows the rapid acquisition of fluorescence images in living cells. It is equipped with RGB, CFP/YFP and far-red filters, incubator with CO2 and temperature control and motorised plate. It also has image deconvolution software.
• Leica SP5 confocal microscope, equipped with resonant scanner, incubation chamber with CO2 and O2 control, and excitation at 405, 488, 561 and 633 nm. –
• Olympus Mitico IX83 TIRF Light Microscope, equipped with Hamamatsu CCD camera, incubator with CO2 control and excitation at 488 and 561 nm.

Award for the Best Scientific Image of the Year 2022 from the SEBBM. SKELETON OF THE IMMUNE RESPONSE. Dra. Noa B. Martín-Cófreces

• User training in handling the Unit’s equipment.

• Advice on sample preparation, experiment design and fluorescent probes, etc.

• Morphometric analysis of cell cultures. Quantification of cytological parameters based on fluorescence intensity measurements.

• Three-dimensional reconstructions. Deconvolution.

• Confocal video microscopy, wide field or TIRF, according to the requirements of cell velocity and viability.

• FRAP and FRET.

Award for the Best Scientific Image of the Year 2022 from the SEBBM. SKELETON OF THE IMMUNE RESPONSE. Dra. Noa B. Martín-Cófreces