citometria_responsable_iis-laprincesa

Contact person

Telephone: +34 915202386 Fax: +34 915202374

Faculty management:
Dr Cecilia Muñoz Calleja
cmunozc@salud.madrid.org

Technical coordinator:
Mr Víctor López Huete
vlopez@salud.madrid.org

The Flow Cytometry and Cell Separation Unit is a laboratory specialised in cell analysis, immunophenotyping and cell sorting techniques.

The Unit has a mixed structure, since the teams and staff serve on the one hand the clinical requests of Hospital de La Princesa, and on the other hand the research groups of the IIS Princesa.

The facilities are located on the 1st floor of the Department of Immunology and Molecular Biology at Hospital Universitario de La Princesa. Incorporated in the REDLAB of the Comunidad de Madrid.

The objective is to provide IIS Princesa researchers with the technology and qualified personnel necessary in flow cytometry and cell separations to conduct biomedical research.

citometria_servicios_iis-laprincesa

  • Training of authorised users in the handling of equipment and marking techniques.
  • Advice on sample preparation, experiment design and interpretation of results.
  • Analysis of cytometric parameters. Immunophenotyping.
  • Feasibility studies. Apoptosis.
  • Separation of cell populations by flow cytometry (Cell Sorting).
citometria_equipamiento_iis-laprincesa
  • Two BD FACSCanto II Analyser Cytometers: one with an automatic sample-feeding rotor, three lasers (488 nm solid state blue laser, 633 nm He-Ne and 405 nm violet laser), allowing eight fluorescences to be worked with simultaneously and two dispersion parameters (FSC and SSC).
  • BD FACSAria Separator Cytometer: Two 488 nm (blue) and 633 nm (red) lasers, with an analysis capacity of up to nine simultaneous parameters (seven fluorescences plus size and complexity). It can separate up to four different populations simultaneously. It offers the possibility of collecting the separate populations in 5 and 15 ml tubes, and microtubes.
  • BD FACSCalibur analyser cytometer: Equipped with two lasers (488 nm and 633 nm). It can simultaneously measure four fluorescences, in addition to size and complexity.
citometria_software_iis-laprincesa
  • BD FacsDiva 6.0 Sofware: allows the acquisition of data both in the FACSAria cell separator and in the FACSCanto II analysers, as well as the subsequent analysis of data manually or in batches. Plots with the possibility of linear, logarithmic and biexponential scales. Data export (FCS 2.0 and FCS 3.0 file format), statistics and templates. 
  • Infinicyt 1.6 (Cytognos): one of the most advanced pieces of software for data integration and multidimensional analysis of flow cytometry files. It has innovative tools that make analysis and interpretation of results faster, easier and more accurate.

The Infinicyt™ software is recommended by the EuroFlow™ consortium.

RATES

SAMPLE TRANSFER

 Service IIS Princesa rate External academic rate External business rate
FACSCanto II cytometer € 5/hour € 70/hour € 90/hour
FACSAria cellular separator € 12/hour € 150/hour € 200/hour