biobanco

Mara Ortega/Sergio Luquero
C/ Diego de León 62
28006 Madrid, Spain

E-mail:
biobanco.hlpr@salud.madrid.org

Telephone:
+ 34 91 520 22 00 ext. – 17526/19527

Opening hours:
Monday to Thursday: 8 am – 5 pm
Friday:8 am – 3 pm

The Biobanco-HUP was authorised by the Comunidad de Madrid in 2013 in accordance with Spanish Royal Degree RD 1716/2011 and since that same year has formed part of the Plataforma Nacional de Biobancos (Spanish National Biobank Platform). Our mission is focused on developing collections of quality biological samples for use by the scientific community. Our fundamental pillars are:

  • To promote the creation of collections in order to adapt them to the needs of researchers.
  • To provide the scientific community with access to biological samples and associated data.
  • To guarantee patients’ rights in terms of donation.
  • To advise researchers on the use and handling of biological samples.
biobanco-iislaprincesa_1The owner and the person responsible for the Biobanco contents is the Fundación de Investigación Biomédica (FIB) at Hospital Universitario de La Princesa. The Chief Scientific Officer is Dr Mara Ortega Gómez, assisted by technician Sergio Luquero Bueno.

According to Spanish Royal Decree RD 10176/20111, it is part of two External Committees (Research Committee of the IIS-IP and the Research Ethics Committee of Hospital de La Princesa). It also has a Collaborative Committee, made up of researchers from the insititution that advises the Biobanco.

pdf-iconCEIm

pdf-icon External Scientific Committee

pdf-icon Scientific Advisory Board

TECHNOLOGY AND EQUIPMENT

In 2010, this department was connected to the new Research Unit located on the 7th floor of our hospital

The Biobanco has two rooms:

SAMPLE RECEPTION AND PROCESSING LABORATORY

The reception and processing of samples is carried out in this laboratory. It has a laminar flow cabin and a CO2 incubator for cell cultures. It also has all the necessary equipment for the processing and aliquoting of such cultures.

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SAMPLE STORAGE AREA

The storage room is equipped with two freezers for storing samples at -80 ºC and -20 ºC. It also has a cell cryopreservation system by means of a “dry” liquid nitrogen tank system which, using a double jacket (filled with liquid nitrogen) around the perimeter of the tank and at its base, ensures a temperature gradient ranging from -190 ºC at the tank mouth to -185 ºC at the bottom of the sample tray. This guarantees a uniform temperature for samples, with no risk to the user as there is no liquid nitrogen in the tank, and full use of its capacity.

As a system available to preserve sample integrity in case of failures in storage devices, the Biobanco has a system of Temperature Probes monitored using real time measurement, thus offering a reliable solution to monitor and record temperature data wirelessly. In case of any abnormality in receiving the measurement, the associated software issues an automatic telephone call to members of the Biobanco to resolve the incident as soon as possible.

Identification of processed and aliquoted samples in our facilities is done using a code generated by the Bio-e-Bank management system. The program integrates the origin of the sample and the coding generated by the program. In addition, at the time of transfer, a third code is generated, thus guaranteeing sample confidentiality and traceability.

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The Biobanco is also an establishment for hosting biological samples for biomedical research purposes, a support platform that offers services related to the handling of biological samples.

TECHNICAL AND SCIENTIFIC ADVICE

  • Creation and management of new collections.
  • Methodology of new collections.
  • Adjustment of laboratory techniques.

MANAGEMENT OF PRIVATE COLLECTIONS

  • Storage of pre-existing collections (-80 ºC, liquid nitrogen).

TISSUES

  • Cryopreservation of tissues.

CELLULAR TECHNIQUES

  • Purification and freezing of PBMCs.
  • Immortalisation of B lymphocytes.

MOLECULAR TECHNIQUES

  • Extraction of DNA from peripheral blood and/or frozen tissue.
  • Serum and plasma isolation

BIOBANK RATE

Download Biobank Rate in PDF

RATE APPLICANT RESEARCH PROFILE
A Researchers members of the IIS-IP
B Researchers not members of the IIS-IP; attached to public institutions
C Researchers not members of the IIS-IP; attached to privateinstitutions

SAMPLE TRANSFER

RATE A RATE B RATE C
DNA aliquot (1 µg) 3,20 € 4,16 € 6,40 €
Serum aliquot (500 µl) 2,14 € 2,78 € 4,28 €
Plasma aliquot (500 µl) 3,30 € 4,29 € 6,60 €
Peripheral blood aliquot (1 ml) 1,07 € 1,39 € 2,14 €
PBMCs aliquot (Ficoll from 5 ml of blood) 9,99 € 12,98 € 19,97 €
Saliva aliquot (200 µl) 3,22 € 4,19 € 6,44 €
Urine aliquot (1 ml) 1,29 € 1,68 € 2,58 €
Tissue (corte tejido congelado OCT) 9,79 € 12,73 € 19,59 €

OTHER SERVICES

RATE A RATE B RATE C
DNA extraction (10 ml of blood) 81,72 € 106,24 € 163,44 €
Separation of mononuclear cells (5-10 ml of blood) 46,98 € 61,08 € 93,97 €
Obtaining plasma aliquots (8-10 ml of blood) 19,80 € 25,73 € 39,59 €
Obtaining serum aliquots (8-10 ml of blood)  21,42 €  27,85 € 42,84 €
Whole blood aliquoting  12,72 €  16,54 € 25,44 €
Storage-80 ºC *  60,00 €  78,00 € 120,00 €
Storage -197 ºC (liquid nitrogen) *  68,00 €  88,40 € 136,00 €

* These rates correspond to the storage for one year of a box with a maximum of 100 tubes. For storage in tubes with 2D coding (only for storage at -80ºC), contact the Biobank.

The rates have been determined based on the total cost of obtaining the sample. The final rate includes the proportional costs of obtaining, processing, storage, maintenance and other expenses of a similar nature related to the samples.

The rates of the services must be signed by both the Principal Investigator and the Biobank Manager.

 

Rates approved by the Board of Trustees of the Biomedical Research Foundation of the Hospital de La Princesa on December 12, 2019

COLECCIÓN PATOLOGÍA ASOCIADA
PROYECTO UROCIENCIAS Patología Oncológica Genitourinaria.
CÁNCER DE TIROIDES Carcinoma Tiroideo Primario.
ADENOMAS PARATIROIDEOS Hiperparatiroidismo Primario con intervención quirúrgica
CONTROLES SANOS Sujetos sin enfermedad conocida
ARTRITIS DE RECIENTE COMIENZO Artritis de reciente comienzo sin filiar
PROYECTO BIOIMID Enfermedades inflamatorias mediadas por el sistema inmune (Artritis Reumatoide, Enfermedad Inflamatoria Intestinal, Esclerosis Múltiples y Psoriasis).
ADULTOS CON SÍNDROME DE DOWN Sujetos adultos con síndrome de Down
TRANSTORNO DEPRESIVO MAYOR Y CONTROLES PAREADOS Depresión Mayor y controles pareados por edad y sexo
INSUFICIENCIA CARDIACA DESCOMPENSADA (NICE) Sujetos con insuficiencia cardíaca descompensada
NEUMONÍA ADQUIRIDA EN LA COMUNIDAD Neumonía adquirida en la comunidad que requiera ingreso hospitalario
ENFERMEDAD POLIQUÍSTICA DEL ADULTO Poliquistosis renal

 

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