Contact person

Staff
Milagros Guerra Rodríguez
Tamara Martín Pozas

Technical manager
Dr María Teresa Rejas Marco
Tel. +34 91 196 45 38/32.
email: mtrejas@cbm.uam.es

Scientific manager:
Dr Germán Andrés Hernández
Tel. + 34 91 196 45 48
E-mail: gandres@cbm.csic.es

The Department of Electronic Microscopy provides scientific and technical support to research groups interested in using electron microscopy techniques for ultra-structural analysis of biological samples and antigen immunodetection. We have a transmission electron microscope (JEM1010, Jeol) and the material required for sample preparation.

infraestructuras-microscopia-electronica-iis-laprincesa

1) Negative staining of macromolecular complexes (viruses, filaments, etc.).

2) Conventional processing of samples in plastic resins.

3) Cryosubstitution and cryoinclusion.

4) Ultramicrotomy of samples embedded in resin.

5) Cryoultramicrotomy.

6) Electronic immunomicroscopy with colloidal gold conjugates.

7) Cryofracture, freeze etching and obtaining Pt-C replicas.

8) Nucleic acid electron microscopy.

DEPARTMENT OF ELECTRONIC MICROSCOPY

C/ Nicolás Cabrera, 1 UAM 28049 – Madrid, Spain

Tel. (+34) 911964532 / 38                                                       Fax. (+34) 911964420

E-mail: sme@cbm.uam.es

 2014 RATES  CBMSO EXTERNAL *
DESCRIPTION CODE PRICE PRICE
Uranyl acetate (ml) (1) ACU € 1.50 € 2.00
Box of grids (50 pieces) BX50 € 13.00 € 18.00
Box of grids (100 pieces) BX100 € 17.00 € 24.00
Box of slides CAP € 12.00 € 17.00
Colloidal gold conjugate (µl) ORO € 2.00 € 2.80
Block cutters COR € 16.00 € 22.00
Semi-fine cuts (carrier) SEM € 5.00 € 7.00
Cryocuts (session) CCO € 70.00 € 98.00
Ethane or propane cryofixation (session) CF2 € 30.00 € 42.00
Cryofracture (expt) CFT € 55.00 € 77.00
Cryosubstitution (1-4 samples) (2) CS4 € 60.00 € 84.00
Cryosubstitution (5-8 samples) CS8 € 90.00 € 126.00
Glass knife CUC € 1.50 € 1.40
Structure fixative (ml) (3) FIE € 1.00 € 1.40
Immune fixative (ml) (4) FIN € 0.50 € 0.70
Glutaraldehyde 25% (ml) GLU € 2.50 € 3.50
In-situ hybridisation (grid) HIS € 5.00 € 7.00
Inclusion in EPON (sample) (5) EPO € 14.00 € 19.00
Immune marking (grid) (6) INM € 4.00 € 5.50
Double immune marking (grid) IM2 € 5.00 € 7.00
EPON syringe (5 ml) JEP € 5.00 € 7.00
M.E. nucleic acid (expt) DNA € 30.00 € 40.00
JEM1010 microscope (hour) MEH € 15.00 € 20.00
Paraformaldehyde (10 ml) PFA € 0.40 € 0.55
Forceps PIN € 40.00 € 56.00
Vents (unit) (7) REJ € 1.00 € 1.40
Pt/C shade SOM € 15.00 € 21.00
Osmium Tetroxide (ml) OSO € 18.00 € 20.00
Negative staining (grid) TNE € 3.00 € 4.20
  1. 2% aqueous solution.
  2. Cryoprotection, vitrification in liquid propane (plunge freezing), dehydration at low temperature and cryo-embedding in Lowicryl resins.
  3. 4% Paraformaldehyde (PFA) + 2% Glutaraldehyde (GT) in pad.
  4. 2% PFA + 0.2% GT or 4% PFA + 0.05 – 0.1 GT in pad.
  5. Includes post-fixation with Osmium, block staining with Uranyl Acetate, dehydration and embedding in TA in Epoxy resin or White LR.
  6. Post-embedding immune marking with colloidal gold conjugates on resin sections or cryosections.
  7. Cu, Ni or Cu/Pd grids covered with Collodion film or Formvar and C shades.

*CBMSO rates will be 100% higher for external users from private organisations